Gibberellin treatment breaks dormancy of radish seeds
Liu Yushi, Ding Jiumin (Lianyungang Vocational and Technical College)
For most seed companies, some radish seeds that have just been recovered from farmers or breeding bases are dormant. The dormant seeds will affect the seed germination rate. According to relevant data, gibberellin has a role in breaking the dormancy of seeds. In this experiment, radish seeds were treated with different concentrations of gibberellin solution at different times to determine the best concentration of gibberellin solution and treatment time to break the radish seed dormancy.
1 test materials and methods
1.1 Test materials
The well-mixed radish dormant sample seeds (11 households for 2 households each, filled into small sachets), 75% gibberellin 2g, and 75% alcohol 1 bottle.
1.2 Treatment
2 g of gibberellin was poured into 2 containers on average, and an adequate amount of alcohol was added to stir to fully dissolve it. One pours 5 kg of water and formulates an approximately 150 mg/kg aqueous gibberellin solution. Another 7 kg of water was poured into about 100 mg/kg gibberellin aqueous solution. Ten samples from each farmer's household were placed in clean water, five of them were placed in a solution of 150 mg/kg, another five were placed in a solution of 100 mg/kg, and the remaining one was used as control water. soak. The treatment time was 2h, 3h, 4h, 8h, 12h. Take 100 seeds of the treated sample seeds and place them evenly on the prepared petri dishes. Place them in an incubator at a temperature of 25 to 30 °C for the germination rate test.
2 Results and Analysis
The treated seeds were placed in an incubator and germination data was recorded on the second day. The germination potential was counted from the 4th day of germination, and the germination rate was recorded on the 10th day. The results are shown in Table 1.
It can be seen from Table 1 that the gibberellin-treated radish dormant seeds have different treatment times and different treatment concentrations, and the seed germination rate and germination potential are different. Seeds of the same farmer's code, whether they were 150 mg/kg or 100 mg/kg gibberellin seeds, had their germination rate and germination potential increased gradually from 2 h after treatment, and the germination rate and germination potential reached the maximum when soaked for 4 h. The value then began to decline; although the germination rate and germination potential were not significantly different between 8h and 12h, none of the seeds that had been soaked for 4 hours had good germination effect. In addition, germination of seeds soaked with gibberellin at a concentration of 150 mg/kg was better than that of soaking at a concentration of 100 mg/kg for the same treatment time. However, the dormant radish seeds treated with clear water as a control did not germinate until 8 h before soaking, and the highest germination rate was only 27% when soaked for 12 h. Both the germination potential and the germination rate were far lower than those treated with gibberellin solution.
3 conclusions
The above experimental results show that the treatment of seeds with a gibberellin aqueous solution at a concentration of 150 mg/kg for 4 h has the best effect on breaking the dormancy of radish seeds and increasing the germination rate. In comparison with seeds treated with water, the seed germination rate is very low and the effect is the worst.Our Inner Ring">Inner Ring is good in quality and competitive in price. We are manufacturer and supplier of Inner Ring following your specific requirement. We are looking forward to your E-mail and establishing cooperative relationship with you! We would provide professional Inner Ring with good services for you!
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