Aflatoxin is very toxic and can destroy the liver tissue of humans and animals. At present, AFB1 assay methods include: thin-layer chromatography, microcolumn screening, high performance liquid chromatography, enzyme-linked immunosorbent assay, etc. Chromatography, microcolumn screening, and high performance liquid chromatography correspond to thin-layer chromatographs, ultraviolet lamps, liquid chromatographs, etc., and have corresponding national regulatory procedures. The enzyme-linked immunosorbent assay corresponds to Aspergillus flavus. Toxin analyzers do not have calibration procedures or calibration specifications. The LISA assay AFB1 has the advantages of advanced technology, high sensitivity, high specificity, high accuracy, good repeatability, fast measurement speed, low cost, low pollution, and the ability to measure large quantities of samples, energy limits, and quantitative determinations at one time.
Aflatoxin analyzer is mainly used in the detection of AFB1 in food and feed products, and consists of light source, monochromator (interference filter), sample cell, detector (silicon photocell) and A/D converter.
In the daily verification or calibration, due to the lack of technical documents such as verification protocols or calibration specifications, the technical indicators of the measurement characteristics of the aflatoxin meter are out of control, which affects the traceability of the measurement performance of the instrument and the accuracy of the instrument. The measured value displayed on the instrument is the absorbance value. The absorbance measurement value of the instrument can be calibrated by using a spectrally neutral filter. Spectral neutral filter absorbance nominal values ​​should be about 0.2, 0.5, 1.0, 1.5, and fixed at 450nm, 490nm wavelength, the extended uncertainty of not more than 0.010 (k = 2). Instrument interference filters can generate monochromatic light of a specific wavelength, and the wavelength accuracy of interference filter peaks can be determined with a Class I or Class II spectrophotometer.
The calibration technique of the aflatoxin analyzer based on enzyme-linked immunosorbent assay has proved that the method is simple and easy to implement, and can achieve the interference wave filter peak wavelength error, the zero drift of the absorbance, the indication value stability, the indication error, and the The comprehensive evaluation of the quantitative performance indicators such as the reproducibility of the aflatoxin analyzer, the indicators can be traced effectively. According to the proposed calibration method, the testing service for aflatoxin analyzer was developed and calibrated on dozens of plate washers successively. Satisfactory results were obtained.
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