Under normal circumstances, we use three wavelengths for protein experiments: 214, 280, and 360, of which 280 is the most widely used. Devices using GE/AKTA can only simulate two-wavelength channels, but the performance is stable but the price is also prohibitive for the average manufacturer. Is there a more cost-effective way? Based on existing products, Brighttech has made a single-wavelength UV detector solution for HY-UVL100 photodiode modules.
Required accessories: Photodiode module + silicon diode + direct fiber / 280nm UV single wavelength light source
1. Photodiode module (using HY- UVL100 ), one for silicon photodiode to provide installation environment, the optical signal into electrical signals, basic data processing, and provide communication interface for the host computer. 
2. Silicon photodiode (HS133-5BQ, from Hamamatsu, Japan), a silicon photodiode, has a wide detection range, high sensitivity, low noise, and a wider signal-to-noise ratio, and has been subject to the detector manufacturer's Favor. 
3, through fiber (HY-FIB100), 2 play a connecting role 
4, xenon lamp ( HY-DL100 ), one (plus 280nm filter)
     It can provide 190~600nm continuous wavelength light, especially in the deep ultraviolet range.
Or HY-LedLight100 series 280nm UV single wavelength light source directly provides 280nm wavelength ultraviolet light 
5, flow cell (HY-FCL50) [0.1 ~ 50mL/min], a
5, UV software ( HY-DataAnalysis ) [integrated data acquisition, processing, preservation, peak integration, quantitative analysis, printing integrated chromatography software platform], or open source test development platform (collection data collection, curve analysis, can Secondary development)
For the light source, we provide two kinds of solutions, need to explain, the following assembly diagram we only use Xenon light source as an example
a. HY-DL100 xenon lamp +280 filter, high cost, but can cover deep ultraviolet (such as 214nm)
b. HY-LedLight100 280nm LED lamp, low cost, no filter, but can not cover the deep ultraviolet (214nm), the lowest only to 265nm
Assembly diagram is as follows
The parameters of the above scheme are based on a single detector and a single diode. The instrument can reach the following parameters:
Absorbance range: ±4AU
Noise: 1×10-5AU
In fact, the HY-UVL100 photodiode module can support two photodiode acquisitions at the same time, and commercial detectors generally use dual optical channels. If we adopt a dual-optical path solution, we will not increase the cost by a large margin, but the detection performance will be greatly improved and the temperature drift will be greatly reduced. So next we introduce the dual-path UV detector solution.
The requirements for accessories and the above are different, 2 and 3, everything else is the same
2. Silicon photodiode (HS133-5BQ, from Hamamatsu, Japan), 2
3, through fiber (HY-FIB100), 1 root, Y fiber, 1 
Assembly diagram is as follows 
Absorbance range tested by dual light path detector: ±4AU
Noise: 0.5×10-5AU
Greatly suppress temperature drift, noise can also be reduced to the original 1/2. For chromatograph manufacturers, we strongly recommend the use of dual-optical path solutions, is also a common practice of several current mature manufacturers (GE \ AKTA \ Shimadzu). And the module supports secondary development, and the sample source code is open source. Users only need to care about the application. If you do not need secondary development, we provide a single-wavelength detector platform for forming the lower position. This platform is compatible with all the above devices. The platform is also seamlessly compatible with our chromatography system . In short, this is a kind of single-wavelength detector scheme that is low-cost, high-efficiency, and can achieve detection effect or international level.
UV Detector, Photo Diode, Agriculture/Food, Single Wavelength Light Source, Control/Automation, Environmental Protection, Biopharmaceutical, Protein Preparation & Purification
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