The relationship between dietary fiber and human health is very close, and people are paying more and more attention to it. Therefore, there is a growing demand for data on dietary fiber in foods. This has certain requirements for the determination of dietary fiber in foods. . Summary Dietary fiber analysis methods are based on chemical reactions and enzymatic reactions that dietary fibers can perform. Historically, important methods for analysis of dietary fiber include non-enzymatic gravimetric methods such as crude fiber, neutral detergent fibers, and acidic detergent fibers. Analytical methods; Enzymatic gravimetric methods There are mainly methods for the determination of total, soluble and insoluble fibres provided by AOAC; the enzymatic methods include enzyme colouring, enzymatic gas chromatography, enzyme high-pressure liquid chromatography and European institutions Various research and application methods for the determination of dietary fiber. Fibrometers are important instruments for fiber measurement and are the only choice for modern scientific research.
Different departments in China have issued a series of national standard methods based on the improvement of dietary fiber analysis methods in different periods. The earliest standard was issued by the Ministry of Health of the People's Republic of China in 1985, GB5009.10-85 (Method for measuring crude fiber in foods). The principle of this method is to remove sugar, starch, pectin, and hemicellulose from water by sulfuric acid. After it was removed, it was treated with alkali to remove protein and fatty acids. The remaining residue was crude fiber. If it contains impurities insoluble in acid and alkali, it can be removed after ashing. This standard is used for the determination of crude fiber content in plant foods. Based on this analytical method, the National Bureau of Standards issued a GB5515-85 Grain and Oil Inspection-Rough Cellulose Assay in 1985 to guide the determination of crude cellulose in commercial grains. In 1987, the Ministry of Commerce issued the GB8310-87 tea coarse fiber assay for the determination of crude fiber in tea. In 1989, the GB10469-89 method for the determination of crude fibres in fruits and vegetables was introduced for the determination of crude fibre content in fruits and vegetables.
GB9822-88 (Grain Insoluble Dietary Fiber Assay) is a measure of insoluble dietary fiber for cereals approved by the Ministry of Commerce in 1988. The principle of the method is that the grain sample is digested with a neutral detergent solution and the residue is thoroughly washed with hot water. A-amylase solution was added to decompose residual bound starch, washed with water and acetone, and dried to obtain insoluble dietary fiber. GB12394-90 (Determination of insoluble dietary fiber in foods), issued by the Ministry of Health in 1990, is a commonly used method in China. The standard stipulates the neutral detergent measurement method for insoluble dietary fiber in foods, and it is applicable to the determination of insoluble dietary fiber in all kinds of plant foods and mixed foods containing plant foods. The minimum detection limit is O.lmg. The principle of the method is that the neutral detergent (the main ingredient is usually sodium dodecyl sulfate) is digested. The sugar, starch, protein, pectin and other substances in the sample are dissolved and removed, and the indigestible residue is insoluble dietary fiber. It mainly includes cellulose, hemicellulose, lignin, keratin, and silica, and includes insoluble ash. The fiber measured by this method is called neutral detergent fiber. If the sample is digested with an acidic detergent (the main component is cetyltrimethylammonium), the measured fiber is called acidic detergent fiber. Neutral washing cellulose is an estimate of the total plant cell wall content, which mainly includes cellulose, hemicellulose and lignin. Acidic detersive fiber is an estimate of the cellulose and lignin content. Based on the difference between the two, the hemicellulose content in the feed can be estimated. The ratio of cellulose, hemicellulose, and lignin in crude fiber is often used to guide the formulation of feed ingredients for pigs.
Compared to the crude fiber assay, the detergency assay recovers all cellulose, insoluble hemicellulose, and lignin as measured by a fiber meter. However, the detergent method can only measure insoluble dietary fiber and cannot measure soluble dietary fiber. With the upgrading of dietary fiber research and the emergence of more and more varieties of dietary fiber, the traditional non-enzymatic one-weight method has shown obvious deficiencies.
Different departments in China have issued a series of national standard methods based on the improvement of dietary fiber analysis methods in different periods. The earliest standard was issued by the Ministry of Health of the People's Republic of China in 1985, GB5009.10-85 (Method for measuring crude fiber in foods). The principle of this method is to remove sugar, starch, pectin, and hemicellulose from water by sulfuric acid. After it was removed, it was treated with alkali to remove protein and fatty acids. The remaining residue was crude fiber. If it contains impurities insoluble in acid and alkali, it can be removed after ashing. This standard is used for the determination of crude fiber content in plant foods. Based on this analytical method, the National Bureau of Standards issued a GB5515-85 Grain and Oil Inspection-Rough Cellulose Assay in 1985 to guide the determination of crude cellulose in commercial grains. In 1987, the Ministry of Commerce issued the GB8310-87 tea coarse fiber assay for the determination of crude fiber in tea. In 1989, the GB10469-89 method for the determination of crude fibres in fruits and vegetables was introduced for the determination of crude fibre content in fruits and vegetables.
GB9822-88 (Grain Insoluble Dietary Fiber Assay) is a measure of insoluble dietary fiber for cereals approved by the Ministry of Commerce in 1988. The principle of the method is that the grain sample is digested with a neutral detergent solution and the residue is thoroughly washed with hot water. A-amylase solution was added to decompose residual bound starch, washed with water and acetone, and dried to obtain insoluble dietary fiber. GB12394-90 (Determination of insoluble dietary fiber in foods), issued by the Ministry of Health in 1990, is a commonly used method in China. The standard stipulates the neutral detergent measurement method for insoluble dietary fiber in foods, and it is applicable to the determination of insoluble dietary fiber in all kinds of plant foods and mixed foods containing plant foods. The minimum detection limit is O.lmg. The principle of the method is that the neutral detergent (the main ingredient is usually sodium dodecyl sulfate) is digested. The sugar, starch, protein, pectin and other substances in the sample are dissolved and removed, and the indigestible residue is insoluble dietary fiber. It mainly includes cellulose, hemicellulose, lignin, keratin, and silica, and includes insoluble ash. The fiber measured by this method is called neutral detergent fiber. If the sample is digested with an acidic detergent (the main component is cetyltrimethylammonium), the measured fiber is called acidic detergent fiber. Neutral washing cellulose is an estimate of the total plant cell wall content, which mainly includes cellulose, hemicellulose and lignin. Acidic detersive fiber is an estimate of the cellulose and lignin content. Based on the difference between the two, the hemicellulose content in the feed can be estimated. The ratio of cellulose, hemicellulose, and lignin in crude fiber is often used to guide the formulation of feed ingredients for pigs.
Compared to the crude fiber assay, the detergency assay recovers all cellulose, insoluble hemicellulose, and lignin as measured by a fiber meter. However, the detergent method can only measure insoluble dietary fiber and cannot measure soluble dietary fiber. With the upgrading of dietary fiber research and the emergence of more and more varieties of dietary fiber, the traditional non-enzymatic one-weight method has shown obvious deficiencies.
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